Coach Paulo Bento slimmed down the original 30-man squad named last Tuesday with FC Porto’s Quaresma, 30, who had been expected to make his return to the national side, failling to make the cut.
Also dropped were Lyon goalkeeper Anthony Lopes, defenders Antunes (Malaga) and Rolando (Inter Milan), midfielders Andre Gomes (Benfica) and Joao Mario (Vitoria Setubal) and striker Ivan Cavaleiro (Benfica).
All those named have already played for Portugal with the least experienced being Braga striker Rafa, who has just one cap, followed by Sporting Lisbon midfielder William Carvalho and Benfica defender Andre Almeida, who have two each.
Portugal have been drawn in Group G at the World Cup and will face Germany on June 15 in Salvador, the United States a week later in Manaus and then Ghana on June 26 in Brasilia.
Portugal will face Mexico in a friendly on June 6 at Gillette Stadium, home of the NFL’s New England Patriots in Foxboro, Massachusetts.
They will take on Ireland on June 10 at MetLife Stadium — home of the Jets and the New York Giants.
Goalkeeperss: Rui Patricio (Sporting), Beto (Sevilla/ESP), Eduardo (Braga)
Defenders: Andre Almeida (Benfica), Bruno Alves (Fenerbahce/TUR), Fabio Coentrao (Real Madrid/ESP), Joao Pereira (Valencia/ESP), Neto (Zenith St-Petersburg/RUS), Pepe (Real Madrid/ESP), Ricardo Costa (Valencia/ESP)
Midfielders: Joao Moutinho (Monaco/FRA), Miguel Veloso (Dynamo Kiev/UKR), Raul Meireles (Fenerbahce/TUR), Ruben Amorim (Benfica), William Carvalho (Sporting)
Strikers: Cristiano Ronaldo (Real Madrid/ESP), Eder (Braga), Helder Postiga (Lazio/ITA), Hugo Almeida (Besiktas/TUR), Nani (Manchester United/ENG), Rafa (Braga), Varela (FC Porto), Vieirinha (Wolfsburg/GER)