The busy Thika Superhighway was among those affected as matatu crews barricaded the road at Pangani, bringing traffic to a halt.
Queues stretched back several kilometres as motorists were caught unawares.
Jogoo Road also experienced similar delays as matatus formed convoys into town and refused to allow other motorists to pass through.
Commuters were forced to walk into the city centre as motorists caught in the chaos stood outside their cars awaiting the police to act.
Traffic Commandant Samuel Kimaru warned that they would move in and restore order.
“We will use all means to clear blocked roads, including force. They know (where Nairobi Governor Evans) Kidero’s office is, they should take their grievance there instead of inconveniencing innocent motorists and other Kenyans,” Kimaru told Capital FM News.
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